D5796

Sigma

 

Dulbecco’s Modified Eagle’s Medium - high glucose

With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, cell culture tested

Synonym:DME, DMEM

Description

ApplicationDulbecco's Modified Eagle's Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
LinkageNeed FBS? We can assist with your serum planning. Please visit the Serum Planner for more information.

Properties

sterilitysterile-filtered
formliquid
total impurities Endotoxin, tested
suitabilitycell culture tested
storage temp.2-8°C

Related Products

Related productF2442, Fetal Bovine Serum

References

referenceDulbecco R, and Freeman G., Plaque production by the polyoma virus Virology 8, 396-397, (1959) Abstract
 Samadikuchaksaraei A, and Bishop AE., Derivation and characterization of alveolar epithelial cells from murine embryonic stem cells in vitro Methods Mol. Biol. 330, 233-248, (2006) Abstract
 Chang C, et al., Mesenchymal stem cells contribute to insulin-producing cells upon microenvironmental manipulation in vitro Transplant. Proc. 39, 3363-3368, (2007) Abstract
 Qu CJ, et al., Effects of glucosamine sulfate on intracellular UDP-hexosamine and UDP-glucuronic acid levels in bovine primary chondrocytes Osteoarth. Cartil. 15, 773-779, (2007) Abstract